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. 2020 Nov 28;12(12):3670. doi: 10.3390/nu12123670

Figure 6.

Figure 6

The original extract of Portuguese blueberries (ARE) and the digested fraction (DIG) attenuate NF-kB activation but not interfere with STAT1 activation, in LPS- and IFN-γ-exposed N9 cells. (A) Cells were pre-incubated with the same concentration of total polyphenols (10 µg/mL) provided from ARE or DIG for 3 h, and then stimulated with LPS and IFN-γ for 2 h. NF-kB activation was evaluated as described in the “Materials and Methods” and expressed as a percentage relative to the activated cells (dark bars). (B) Cells were incubated with LPS and IFN-γ for 15 min, 30 min, 1 h and 2 h. STAT1 activation was evaluated as described in the “Materials and Methods” and expressed as a percentage relative to the control cells (non-stimulated cells). Values are mean ± SEM of at least three different experiments, in duplicate. *** p < 0.001 vs. Control and # p < 0.05 and ## p < 0.01 vs. LPS and IFN-γ.