Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 1;18(12):609. doi: 10.3390/md18120609

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Functional kinome profiling of serine/threonine kinases (STKs). 22Rv1 cells were treated with DBF for 2 h; proteins were extracted and analyzed using STK-PamChip^® (sequence-specific peptide phosphorylation assay) and anti-phospho-STK antibodies. (A), Microphotographs of STK-PamChip^®. The generated data is represented as heat-map plot (B), box plots (C), or volcano-plot (D). Red dots indicate peptide substrates having significantly increased phosphorylation in comparison to control samples (log₂(p) > 1.3, dotted line, (D)). (E), Upstream analysis of the treatment-affected kinases in 22Rv1 cells. Normalized kinase statistic > 0 indicates higher kinase activity in DBF-treated cells; specificity score > 1.3 indicates statistically significant changes.