Figure 7.

Analysis of the effect on DBF on AR signaling. (A), 22Rv1 cells were co-treated with DBF in combination with enzalutamide for 48 h. The viability was measured using the MTT assay and the effect of the drug combination (synergism/additive effect/antagonism) was calculated and visualized using SynergyFinder 2.0 software and a ZIP reference model. Red regions indicate synergism; white—additive effect; green—antagonism. (B), Effect of DBF on the expression of several proteins involved in the androgen receptor signaling following 72 h of treatment. Protein expression was accessed using Western blotting. β-actin was used as a loading control.