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. 2020 Nov 27;12(12):3541. doi: 10.3390/cancers12123541

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© 2020 by the authors.

2020 International Agency for Research on Cancer (IARC); Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the terms of the Creative Commons Attribution IGO License (CC BY) (http://creativecommons.org/licenses/by/3.0/igo/legalcode), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In any reproduction of this article there should not be any suggestion that IARC or this article endorse any specific organisation or products. The use of the IARC logo is not permitted. This notice should be preserved along with the article’s original URL.

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Linearity between the input Mutant Allelic Fractions (MAFs) and the experimentally determined MAFs for the TERT C228T assay. The theoretical percentage of MAFs from serially diluted C228T mutated template DNA into wild-type DNA was plotted against the experimentally determined ddPCR MAFs for 5 ng, 10 ng, 20 ng and 40 ng.