Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 27;12(12):3541. doi: 10.3390/cancers12123541

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

2020 International Agency for Research on Cancer (IARC); Licensee MDPI, Basel, Switzerland. This is an open access article distributed under the terms of the Creative Commons Attribution IGO License (CC BY) (http://creativecommons.org/licenses/by/3.0/igo/legalcode), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. In any reproduction of this article there should not be any suggestion that IARC or this article endorse any specific organisation or products. The use of the IARC logo is not permitted. This notice should be preserved along with the article’s original URL.

PMC Copyright notice

Examples of 2D scatterplots obtained from TERT promoter mutation ddPCR assays in representative samples. Assays testing for C228T (a), C250T (b), C228A (c), and CC242-243TT (d) mutations are displayed in four examples of mutated samples (left panels) and in four examples of wild-type samples (right panels). In the left panel, fluorescent probes (FAM) detect respective mutations as exemplified by the count of droplets with mutated alleles, while in the right panel, wild- type samples do not show any positive droplets (FAM) above the threshold line but show droplets with HEX fluorescence associated with wild-type probes. The pink lines are the thresholds for channel 1 (mutated probe) and channel 2 (wild-type probe) for the ddPCR mutation assays.