Table 1.
Performance of urinary TERT promoter mutations in detecting UC by ddPCR and UroMuTERT Assays.
| C228T or C250T Mutations | ddPCR Assays | UroMuTERT Assay | ||||
|---|---|---|---|---|---|---|
| DIAGURO Cohort | PORTO Cohort | DIAGURO Cohort a | PORTO Cohort a | DIAGURO Cohort b | PORTO Cohort b | |
| US cfDNA or UP DNA | UP DNA | US cfDNA or UP DNA | UP DNA | US cfDNA or UP DNA | UP DNA | |
| Total Number analyzed | (n = 181) | (n = 99) | (n = 181) | (n = 99) | (n = 187) | (n = 100) |
| True Positive-no | 79 | 33 | 80 | 32 | 81 | 33 |
| True Negative-no | 85 | 50 | 88 | 50 | 89 | 50 |
| False Positive-no | 7 | 0 | 4 | 0 | 5 | 0 |
| False Negative-no | 10 | 16 | 9 | 17 | 12 | 17 |
| Sensitivity (95% CI)-% | 86.8 (80.3–94.5) | 67.4 (52.5–80.1) | 90.7 (83.1–95.7) | 65.3 (50.4–78.3) | 87.1 (78.6–93.2) | 66.0 (51.2–78.8) |
| Specificity (95% CI)-% | 92.4 (85.0–96.9) | 100.0 (92.9–100.0) | 95.6 (89.2–98.8) | 100.0 (92.9–100.0) | 94.7 (88.0–98.3) | 100.0 (92.9–100.0) |
| Accuracy (95% CI)-% | 91.3 (86.2–95.0) | 90.2 (82.6–95.3) | 93.1 (88.5–96.3) | 89.6 (81.8–94.8) | 92.4 (90.6–94.0) | 89.8 (87.8–91.6) |
a: Data from Avogbe et al. [11], with the samples screened with both ddPCR and UroMuTERT assays. Samples with not enough DNA or ddPCR data were excluded for a precise comparison of the performance of the two methods (n = 6 from the DIAGURO series and n = 1 from the IPO-PORTO cohort). b: Original data from Avogbe et al. [11].