Table 2.
Interrater reliability of ddPCR and UroMuTERT assays in detecting urinary TERT promoter mutations in a large series of serial urine samples from follow-up BC cases.
| Sample Type | UroMuTERT-MUT | UroMuTERT-WT | Kappa Coefficient | ||
|---|---|---|---|---|---|
| ddPCR-MUT | ddPCR-WT | ddPCR-MUT | ddPCR-WT | ||
| n | n | n | n | ||
| All Follow-up cases (UP cellDNA and US cfDNA) | 173 | 10 | 15 | 196 | 0.87 |
| All US cfDNA Follow-up cases | 86 | 3 | 9 | 123 | 0.89 |
| All UP cellDNA Follow-up cases | 87 | 7 | 6 | 73 | 0.85 |
| Cases US/UP follow-up with MAF < 2% ddPCR | 64 | 10 | 14 | 73 | 0.78 |
| Cases US/UP follow-up with MAF > 2% ddPCR | 109 | 0 | 1 | 123 | 0.99 |
| Cases US/UP follow-up with MAF < 1% ddPCR | 18 | 7 | 4 | 73 | 0.70 |
| Cases US/UP follow-up with MAF > 1% ddPCR | 155 | 3 | 11 | 123 | 0.90 |
| Cases US/UP follow-up with MAF < 0.5% ddPCR | 3 | 7 | 3 | 73 | 0.32 |
| Cases US/UP follow-up with MAF > 0.5% ddPCR | 170 | 3 | 12 | 123 | 0.90 |
US: Urine Supernatant. UP: Urine Pellet. MAF: Mutant Allelic Fraction.