Figure 7.

A schematic representation of a translesion synthesis (TLS) model in Arabidopsis. Replicative polymerase complex (either pol δ or pol ε) are blocked by a CPD or 6-4 PP DNA lesion (a). PCNA is monoubiquitynated (b). TLS polymerase which has a strong affinity to monoubiquitinated PCNA is recruited and replaces replicative polymerase complex. AtREV1 (c) and AtPOLη (d) are preferentially recruited to CTD and 6-4 PP, respectively. AtREV1 recruits another polymerase such as AtPOLζ, which can add nucleotides regardless the lesion, but is error-prone (e). AtPOLη synthesis is error free (f).