Table 2.

Results of the regression analysis of predicted quadratic polynomial models for each of the response variables of chickpea sprouts.

	Regression Parameter Coefficients
	Responses Variables 1
Parameter	TPC (mg GAE/100 DW)	TFC (mg CE/100 DW)	AOX (µmol TE/100 g DW)
	Coded Values	Coded Values	Coded Values
Intercept
β0	81.1	+20.6	+1565.1
Linear
β1, H2O2 concentration (X1)	+7.04	+2.87	+155.4
β2, Germination time (X2)	+27.43	+3.86	+360.2
Quadratic
β11, H2O2 concentration ($X_1^2$)	NS	−1.73	NS
β22, Germination time ($X_2^2$)	+9.94	+2.06	NS
Interactive
β12, H2O2 concentration x Germination time (X1×2)	+8.14	NS	+110.2
p-value for model	<0.0001	<0.0001	<0.0001
R2	0.956	0.967	0.933
R2ajusted	0.925	0.947	0.886
p-value for lack of fit	0.138 NS	0.191 NS	0.726 NS
CV (%)	8.3	5.1	4.8
Adequate precision (PRESS)	17.0	23.8	14.1
Responses	Predicted Models	Predicted Values	Experimental Values 2
TPC	$Y_{TPC}\mathsf{=}\mathsf{81.1}+\mathsf{7.04}{\mathit{X}}_{\mathsf{1}}\mathsf{+}\mathsf{27.43}{\mathit{X}}_{\mathsf{2}}\mathsf{+}\mathsf{9.94}{\mathit{X}}_{\mathsf{1}}^{\mathsf{2}}\mathsf{+}\mathsf{8.14}{\mathit{X}}_{\mathsf{1}}{\mathit{X}}_{\mathsf{2}}$	129.47	138.8 ± 2.01
TFC	$Y_{TFC}\mathsf{=}\mathsf{20.6}\mathsf{+}\mathsf{2.87}{\mathit{X}}_{\mathsf{1}}\mathsf{+}\mathsf{3.86}{\mathit{X}}_{\mathsf{2}}\mathsf{-}\mathsf{1}\mathsf{,}\mathsf{73}{\mathit{X}}_{\mathsf{1}}^{\mathsf{2}}\mathsf{+}\mathsf{2.06}{\mathit{X}}_{\mathsf{2}}^{\mathsf{2}}$	27.86	27.8 ± 1.32
AOX	$Y_{AOX}\mathsf{=}\mathsf{1}\mathsf{,}\mathsf{565.1}\mathsf{+}\mathsf{155.4}{\mathit{X}}_1\mathsf{+}\mathsf{360.2}{\mathit{X}}_{\mathsf{2}}\mathsf{+}\mathsf{110.2}{\mathit{X}}_{\mathsf{1}}{\mathit{X}}_{\mathsf{2}}$	2219.4	2347.2 ± 133

¹ Total phenolic content (TPC) Total flavonoid content (TFC) Antioxidant activity (AOX); NS, Not significant at p < 0.05; ² Mean ± standard deviation of three replicates from experiments to optimal germination condition (30 mM H₂O₂, 72 h).