. 2020 Dec 3;10(12):2282. doi: 10.3390/ani10122282

Table 1.

Primer information of PCR amplification for long-chain acyl-CoA synthetase 1 (ACSL1) genes.

Primer Name	Primer Sequences (5′→3′)	Size(bp)	Exon	Position	Tm (°C)
P1	F1: AACCCAGCGTGACCTGTTTCACCAG	963	5’UTR + Exon 1	−484~+479	69
P1	R1: ATGAGCCTCTGCTCCGTGTGTAACG	963	5’UTR + Exon 1	−484~+479	69
P2	F2: GTCCATGCAGCAAACACTCACCC	1070	Exon 2,3 + Intron 2,3	+14,601~+15,648	64
P2	R2: CAACCTACAGAGGCTCCAGAAA	1070	Exon 2,3 + Intron 2,3	+14,601~+15,648	64
P3	F3: ACTGGGCAAGTGTTTTGTTCATTAG	1034	Exon 5,6 + Intron 5,6	+21,364~+22,373	63
P3	R3: TCGCTCAGTCATGTCCGACTCTTAG	1034	Exon 5,6 + Intron 5,6	+21,364~+22,373	63
P4	F4: TCAGCTTGAACTGACTTGATGTGAC	1382	Exon 7–9 + Intron 7–9	+24,637~+25,994	64
P4	R4: ATAGTCCGGGCCTAACATGATGGTG	1382	Exon 7–9 + Intron 7–9	+24,637~+25,994	64
P5	F5: AAGTCCTGCATGGATTTACTTTGTC	485	Exon 10 + Intron 10	+28,070~+28,530	63
P5	R5: GAACTGCCTACGGGGAAGATGG	485	Exon 10 + Intron 10	+28,070~+28,530	63
P6	F6: AAGCCACATTCCCTAGTTGCTG	1028	Exon 11 + Intron 11	+30,241~+31,247	60
P6	R6: GCACTATGAAAGTGGAGGCATC	1028	Exon 11 + Intron 11	+30,241~+31,247	60
P7	F7: TTCCAGTTTCTCCACATCTTCAC	1291	Exon 12,13 + Intron 12,13	+32,505~+33,773	59
P7	R7: ACACATCCGAAGAAAAGAAGGG	1291	Exon 12,13 + Intron 12,13	+32,505~+33,773	59
P8	F8: GGGTCTTTATCCCTTCAGAGGC	1291	Exon 19 + 3’UTR	+42,584~+43,853	60
P8	R8: CCTATGTCCTAGAATTTTGGCTTG	1291	Exon 19 + 3’UTR	+42,584~+43,853	60