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. 2020 Dec 3;12(12):1383. doi: 10.3390/v12121383

Figure 1.

Figure 1

Morphological characteristics and dsRNA content of strains YC81-2 and P131. (A) Colony morphology of strain YC81-2 and P131 on PDA was observed at 7 and 9 days after inoculation (dai). (B) The growth rate was calculated by recording the colony diameters of the two strains at 3, 5, 7 and 9 dai, which showed that there was no significant difference between these two strains. Three independent replicates were performed. The values are means ± S.E. NS indicates no significance (p < 0.01, one-way ANOVA). (C) Hyphal morphology of strains YC81-2 and P131. Both strains were incubated at 28 °C on PDA covered with cellophane for 5 days, and the hyphal morphology was observed by a light microscope. Scale bars shown in the pictures are 10 µm. The hyphal tips of the two strains showed no differences from each other. (D) Agarose gel electrophoresis analysis of dsRNA extracted from the strains YC81-2 and P131. dsRNA samples were loaded into a 1.5% agarose gel. Multiple bands were observed in the dsRNA sample of YC81-2.