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. Author manuscript; available in PMC: 2020 Dec 25.
Published in final edited form as: Nature. 2008 Jun 15;454(7203):528–532. doi: 10.1038/nature07034

Figure 4 |. Smad3 shRNA rescues responses of satellite cells in old niche in vivo.

Figure 4 |

Lentiviral-infected muscle (control transduction (Tdxn), Smad3 shRNAs, non-target shRNA) was analysed 5 days after injury. a, Haematoxylin and eosin 10-μm cryosections of Smad3 shRNA-1; quantified in b; n = 15, *, **P ≤ 0.05; individual Smad3 shRNA-2, −3 data in Supplementary Fig. 10a; immunodetection of eMyHC myofibres with BrdU+ nuclei. Scale bar, 100μm; O, old; Y, young. c, Isolated satellite cells analysed by western blot for p15, p16, p21 and p27; quantified in d; *P ≤ 0.05, compared with non-target shRNA, control tdxn; data are means ± s.d., n = 3.