Skip to main content
. 2020 Dec 3;12(12):3626. doi: 10.3390/cancers12123626

Figure 4.

Figure 4

Inhibition of E2F4as promote apoptosis of ovarian cancer cells. (A) Cell cycle was arrested in the G1 phase after transfection of siE2F4as in ovarian cancer cells. (B) Ovarian cancer cells were transfected with siNC, siE2F4as, and control in triplicate for 48 h to detect cell apoptosis using FITC Annexin V apoptosis kit. (C) Levels of apoptosis in siE2F4as analysed using FACS Canto II. Data expressed as mean ± standard deviation; ** p < 0.01, *** p < 0.001 vs. control. (D) Protein lysates obtained from SKOV3 and OVCA429 cells 48 h after transfection with E2F4as siRNA (30 nM) or negative control. Levels of proteins in apoptosis-associated gene analysed using Western blotting. Data are representative of three independent experiments.