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. 2020 Dec 3;8(4):731. doi: 10.3390/vaccines8040731

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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rTgH2A1 induced the apoptosis of murine Ana-1 cells. Ana-1 cells were preincubated with different concentrations (0, 5, 10, 20, 40, and 80 μg/mL) of rTgH2A1 for 48 h. Results were evaluated using one-way ANOVA analysis followed by Dunnett’s test and expressed as mean ± standard deviation of three independent experiments. *** p < 0.001 compared with the blank group or control group. Effect of rTgH2A1 on the early (a) and late (b) stage apoptosis of murine Ana-1 cells. Cells stained with Annexin V and PI were measured with flow cytometry (c).

rTgH2A1 induced the apoptosis of murine Ana-1 cells. Ana-1 cells were preincubated with different concentrations (0, 5, 10, 20, 40, and 80 μg/mL) of rTgH2A1 for 48 h. Results were evaluated using one-way ANOVA analysis followed by Dunnett’s test and expressed as mean ± standard deviation of three independent experiments. *** p < 0.001 compared with the blank group or control group. Effect of rTgH2A1 on the early (a) and late (b) stage apoptosis of murine Ana-1 cells. Cells stained with Annexin V and PI were measured with flow cytometry (c).