Figure 6.

rTgH2A1 induced cytokine secretion of the murine macrophages in vitro. Preincubated with different concentrations (0, 5, 10, 20, 40, and 80 μg/mL) of rTgH2A1 for 48 h, cytokine secretion was determined by commercial kits based on the double antibody sandwich method. The Ana-1 cells in the blank groups were treated with PBS while the control group was treated with his-tagged protein. Results were evaluated using one-way ANOVA analysis followed by Dunnett’s test, and values were displayed as mean ± standard deviation from three independent experiments. ** p < 0.01, and *** p < 0.001 compared with the blank group or control group. Effect of rTgH2A1 on the TNF-α (a), IL-1β (b), TGF-β1 (c), and IL-10 (d) secretion of murine Ana-1 cells was determined.