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Cytokine production. Double antibody sandwich ELISA kits were used to determine the level of IFN-γ (a), IL-4 (b), IL-10 (c), and IL-17 (d) in sera from animals collected at weeks 0, 2, and 4. Results were evaluated using one-way ANOVA analysis followed by Dunnett’s test, and values were shown as mean ± standard deviation of pg/mL. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with the blank group or his-tagged protein group.
