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. 2020 Dec 3;9(12):1221. doi: 10.3390/antiox9121221

Figure 5.

Figure 5

Inhibitory Effect of PGFE through by HO-1 on PG-LPS induced pro-inflammatory cytokines. The HPDL cells were pretreated with the indicated concentration of PGFE (200 μg/mL) for 6 hr and then incubated with PG-LPS for 24 hr. (A) The production of pro-inflammatory cytokines interleukin (IL)-6, IL-1β and tumor necrosis factor-α (TNF-α) were determined by ELISA kit assay. (B) The mRNA levels of pro-inflammatory cytokines genes and il-6, il-1β, and TNF-α genes were determined by real-time PCR analysis. The results were normalized to gapdh expression. * p < 0.05 vs. only LPS treated group; # p < 0.05 vs. LPS + PGFE treated group.