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. 2020 Dec 3;9(12):1221. doi: 10.3390/antiox9121221

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of HO-1 expression by PGFE on osteogenic induction. (A) The PDL cells were pretreated with or without the indicated concentration of tin protoporphyrin IX (SnPP), then after, PGFE (200 μg/mL) for 6 h and then incubated with lipopolysaccharides (LPS) for 7 days. (B) The protein expressions were confirmed by Western blot analysis. (C) The mRNA levels of alp, col1, opn, and runx2 were measured by real-time PCR. The results were normalized to gapdh or β-actin expression. * p < 0.05 vs. only LPS treated group; ^# p < 0.05 vs. LPS + PGFE treated group. Scale bars denoted 20 μm.