Table 3.

Enzymes activities (Units/mL), total antioxidant capacity, and oxidative status biomarkers in blood plasma and milk of ewes fed the two diets (Control, n = 8 and ActiSaf, n = 8) at two sampling times.

	Diets (D)			Sampling Time (T) in Weeks			Effect *
	Control	ActiSaf	SEM †	3	6	SEM †	D	T	DxT
Blood plasma
GST	0.150	0.132	0.011	0.148	0.134	0.011	0.282	0.336	0.903
GR	0.067	0.076	0.002	0.067	0.076	0.002	0.004	0.003	0.458
GSH-Px	0.398	0.423	0.025	0.353	0.468	0.021	0.476	0.000	0.847
SOD	18.544	20.304	0.987	18.409	20.440	1.170	0.227	0.301	0.427
CAT	4.969	5.008	0.146	4.893	5.084	0.131	0.855	0.255	0.477
FRAP	0.641	0.764	0.045	0.675	0.730	0.036	0.099	0.230	0.409
ABTS	36.888	33.045	0.497	35.577	34.356	0.617	0.000	0.246	0.195
MDA	0.601	0.566	0.067	0.604	0.562	0.055	0.729	0.497	0.304
PC	4.065	4.015	0.085	3.886	4.193	0.110	0.685	0.114	0.981
Milk
LPO	0.504	0.602	0.059	0.628	0.478	0.047	0.265	0.006	0.467
SOD	3.677	3.569	0.173	3.577	3.669	0.157	0.664	0.660	0.859
CAT	29.284	32.359	3.564	33.558	28.085	2.836	0.552	0.054	0.750
FRAP	3.013	3.090	0.179	2.855	3.247	0.158	0.766	0.078	0.445
ABTS	37.563	43.85	1.014	40.254	41.158	1.057	0.001	0.572	0.726
MDA	0.363	0.369	0.018	0.363	0.369	0.015	0.843	0.693	0.687
PC	2.916	2.877	0.106	2.925	2.867	0.108	0.801	0.718	0.541

^† SEM = Standard error of the mean. GST: Glutathione transferase. GR: Glutathione reductase. SOD: Superoxide dismutase. GSH-Px: Glutathione peroxidase. CAT: Catalase. ABTS: 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (inhibition%). FRAP: Ferric Reducing Ability of Plasma (μΜ ascorbic acid). MDA: Malondialdehyde (μΜ MDA). PCs: Protein carbonyls (nmol/mL). LPO: Lactoperoxidase. Significance level below 0.05 indicates significant difference. * Effect: The dietary treatment (D), time (T), and the interaction between dietary treatment × time (DxT) effects were analyzed by ANOVA using a general linear model (GLM) for repeated measures and posthoc analysis was performed when appropriate using Tukey’s multiple range test.