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. 2020 Dec 3;9(12):440. doi: 10.3390/biology9120440

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Results of genome screening. (A) Changes in taxonomy at the species level as a result of Genome Taxonomy Database (GTDB) reclassification; (B) the rRNA operon status of genomes used in this analysis; (C) differences from the previous analysis. Changes in taxonomy and operon status were indicated. * The group “unlinked → mixed” contained 13 “reclassified in genus.” (D,E) Distribution of rRNA gene copy numbers in a genus with a partially unlinked rRNA operon. The number of linked, mixed, and unlinked genomes for each genus was displayed on the x-axis, and each genome was plotted with green, yellow, and red dots. The thick line of the box represents the median, the line at the end of the box represents the first and third quartiles, and whiskers are 1.5× interquartile ranges. Dots that do not overlap whiskers are outliers. The number of 16S and 23S rRNA genes verified by in silico PCR was used. (D) 16S rRNA and (E) 23S rRNA.