Table 3.
Specific primers used for PCR amplification of OXA carbapenemase genes.
| Gene | Primer Sequences (5′ to 3′) | Product Size (bp) | |||
|---|---|---|---|---|---|
| Forward | Tm (°C) | Reverse | Tm (°C) | ||
| Class D | |||||
| oxacillinases | |||||
| blaoxa-23-like | GAT CGG ATT GGA | 60 | ATT TCT GAC CGC | 56 | 501 bp |
| GAA CCA GA | ATT TCC AT | ||||
| blaoxa-24/40-like | TAA TGC TTT GAT | 60 | AGT TGA GCG | 58 | 246 bp |
| CCC TTA AA | CAT CTT GG | ||||
| blaoxa-51-like | TAA TGC TTT GAT | 58 | TGG ATT GCA CTT | 58 | 353 bp |
| CGG CCT TG | CAT CTT GG | ||||
| blaoxa-58-like | AAG TAT TGG GGC | 60 | CCC CTC TGC GCT | 64 | 599 bp |
| TTG TGC TG | CTA CAT AC | ||||