Figure 1.

Induction of EMT in cells with acquired resistance to WZ4002 or osimertinib. (A) Cells were treated with the indicated doses of WZ4002 or osimertinib for 72 h, and cell viability was determined by an MTT assay. IC₅₀ values were calculated with GraphPad software through three independent experiments. (B) H1975 parental cells and the resistant cell lines (H1975/WR and H1975/OR) were evaluated for morphologic changes that were consistent with EMT using a light microscope. (C) EGFR signaling and EMT-related molecules were analyzed by western blotting. (D,E) Cells were seeded onto either collagen or Matrigel-coated polycarbonate filters to determine their migratory and invasive potentials, respectively. Cells were incubated in modified Boyden chambers for 24 h, and the cells that penetrated the filter were stained and counted using a light microscope. Experiments were conducted in triplicate. The bars represent the standard deviations. *** P < 0.0005 compared with H1975 cells.