Table 3.
Genes analyzed by semiquantitative real-time polymerase chain reaction using TaqMan Gene Expression technology (A) or RealTime Ready Assay probes from Roche (B). Gene, GenBank accession number and sequence or assay ID for each primer used in the study are shown.
| (A) | ||
| Gene | GenBank Accession no. | Assay ID (TaqMan®) |
| SDHA | NM_004168.3 | Hs00188166_m1 |
| CD27 | NM_001242.4 | Hs00154297_m1 |
| hTERC | NR_001566.1 | Hs03454202_s1 |
| CDKN2A (p16) | NM_000077.4 | Hs00923894_m1 |
| PDCD1 (PD1) | NM_005018.2 | Hs01550088_m1 |
| KLRK1 (NKG2D) | NM_013431.2 | Hs00255338_m1 |
| B3GAT1 (CD57) | NM_018644.3 | Hs01024500_m1 |
| TNFSF11(RANKL) | NM_003701.3 | Hs00243522_m1 |
| (B) | ||
| Gene | GenBank Accession No. | Assay ID (Roche) |
| GAPDH | NM_002046.7 | 141139 |
| VIPR1 (VPAC1) | NM_004624.4 | 104081 |
| VIPR2 (VPAC2) | NM_003382.5 | 148318 |
B3GAT1: β-1,3-glucuronyltransferase 1; CDKN2A: cyclin-dependent kinase inhibitor 2A; GAPDH: Glyceraldehyde-3-Phosphate Dehydrogenase; hTERC: human telomerase RNA component; KLRK1: killer cell lectin like receptor K1; NKG2D: natural killer group 2 member D; PDCD1: programmed cell death 1; RANKL: receptor activator of nuclear factor-kappa B ligand; SDHA: succinate dehydrogenase complex flavoprotein subunit A; TNFSF11: TNF Superfamily member 11. In the event that the name of the gene does not match the protein, it will appear in parentheses.