Table 1.
Boar sperm parameters assessed with flow cytometry (FC) and computer-assisted sperm analysis (CASA).
| Active/Compromised Sperm Ratio |
Flow Cytometry Assessment | CASA Assessment | |||
|---|---|---|---|---|---|
| Viability (%) |
Acrosome Integrity (%) |
Mitochondrial Integrity (%) |
Motile Sperm (%) |
Progressive Sperm (%) |
|
| 0/10 | 0.40 ± 0.11 a | 0.23 ± 0.18 a | 5.50 ± 1.87 a | 0.00 ± 0.00 a | 0.00 ± 0.00 a |
| 2/8 | 12.47 ± 1.32 b | 9.88 ± 1.17 b | 16.62 ± 2.30 b | 18.95 ± 1.50 b | 8.18 ± 0.52 b |
| 4/6 | 24.03 ± 2.21 c | 18.83 ± 0.98 c | 29.86 ± 1.83 c | 35.35 ± 1.92 c | 16.17 ± 0.64 c |
| 6/4 | 36.50 ± 3.03 d | 30.10 ± 1.31 d | 44.60 ± 1.75 d | 52.05 ± 2.39 d | 23.72 ± 1.33 d |
| 8/2 | 52.33 ± 2.73 e | 46.03 ± 1.56 e | 58.78 ± 0.65 e | 67.37 ± 2.63 e | 32.92 ± 1.18 e |
| 10/0 | 72.05 ± 3.06 f | 62.65 ± 1.47 f | 73.80 ± 0.95 f | 81.37 ± 2.42 f | 40.75 ± 2.02 f |
Values are expressed as means ± SEM from 36 samples in 6 independent experiments. a–f indicates significant differences (p < 0.01) between different active/compromised ratios within the same column of a tested parameter. Statistical analysis was performed using one-way ANOVA.