Table 2.
Pearson’s correlation coefficients between the 2-[2-methoxy-4-nitrophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H-tetrazolium (WST-8) reduction rate and boar sperm parameters in different sperm concentrations and incubation times.
| Parameters | Correlation Coefficients (r) | |||
|---|---|---|---|---|
| Sperm Concentration (106 cells/mL) | ||||
| 300 | 150 | 75 | 37.5 | |
| 10 min | ||||
| Viability (%) | 0.48 | −0.61 | −0.18 | −0.16 |
| Acrosome integrity (%) | 0.54 | −0.49 | −0.31 | −0.16 |
| Mitochondrial integrity (%) | 0.30 | −0.86 * | −0.62 | −0.40 |
| Motile sperm (%) | 0.52 | −0.46 | −0.23 | −0.15 |
| Progressive sperm (%) | 0.51 | −0.45 | −0.22 | −0.16 |
| 20 min | ||||
| Viability (%) | 0.81 * | −0.60 | −0.06 | −0.06 |
| Acrosome integrity (%) | 0.88 * | −0.49 | −0.20 | −0.12 |
| Mitochondrial integrity (%) | 0.81 * | −0.90 * | −0.58 | 0.41 |
| Motile sperm (%) | 0.81 * | −0.48 | −0.11 | −0.11 |
| Progressive sperm (%) | 0.78 * | −0.49 | −0.10 | −0.10 |
| 30 min | ||||
| Viability (%) | 0.87 * | −0.28 | 0.10 | −0.10 |
| Acrosome integrity (%) | 0.92 * | −0.19 | −0.04 | −0.08 |
| Mitochondrial integrity (%) | 0.85 * | −0.88 * | 0.42 | 0.23 |
| Motile sperm (%) | 0.84 * | −0.20 | 0.08 | −0.04 |
| Progressive sperm (%) | 0.82 * | −0.21 | 0.09 | −0.03 |
| 40 min | ||||
| Viability (%) | 0.89 * | −0.04 | 0.32 | 0.08 |
| Acrosome integrity (%) | 0.91 * | 0.05 | 0.30 | 0.09 |
| Mitochondrial integrity (%) | 0.85 * | −0.89 * | 0.53 | 0.45 |
| Motile sperm (%) | 0.82 * | 0.03 | 0.45 | 0.12 |
| Progressive sperm (%) | 0.82 * | 0.02 | 0.45 | 0.12 |
| 50 min | ||||
| Viability (%) | 0.89 * | 0.36 | 0.34 | 0.39 |
| Acrosome integrity (%) | 0.89 * | 0.42 | 0.42 | 0.56 |
| Mitochondrial integrity (%) | 0.85 * | −0.47 | 0.34 | 0.60 |
| Motile sperm (%) | 0.80 * | 0.36 | 0.50 | 0.59 |
| Progressive sperm (%) | 0.81 * | 0.36 | 0.49 | 0.60 |
| 60 min | ||||
| Viability (%) | 0.88 * | 0.51 | 0.61 | 0.75 * |
| Acrosome integrity (%) | 0.87 * | 0.56 | 0.68 | 0.78 * |
| Mitochondrial integrity (%) | 0.84 * | −0.09 | 0.85 * | 0.72 |
| Motile sperm (%) | 0.78 * | 0.50 | 0.74 * | 0.84 * |
| Progressive sperm (%) | 0.79 * | 0.50 | 0.74 * | 0.85 * |
WST-8 reduction rate = ΔOD at 450 nm. A standard semen/cell-counting kit-8 (CCK-8®) solution mixture of 100/10 (μL, v/v) was used. Data presented are the average values collected from 36 samples from 6 independent experiments, for each value, 6 measurements (n = 6) were performed. Asterisks (*) indicate that the correlation coefficient value differs significantly (p < 0.01) from zero. Bold font represents positive correlation.