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. 2020 Dec 4;10(12):2293. doi: 10.3390/ani10122293

Table 3.

Pearson’s correlation coefficients between the WST-8 reduction rate and boar sperm parameters in different ratios of semen/cell-counting kit-8 (CCK-8®) solution mixtures (v/v).

Correlation Coefficients (r)
Sperm Concentration (106 cells/mL) 300 150
Semen/CCK-8® (v/v, μL) 200/20 200/10 200/20 200/10
10 min
Viability (%) 0.88 * 0.90 * 0.42 −0.14
Acrosome integrity (%) 0.87 * 0.88 * 0.41 −0.12
Mitochondrial activity (%) 0.83 * 0.84 * 0.44 −0.09
Motile sperm (%) 0.78 * 0.81 * 0.49 0.01
Progressive sperm (%) 0.81 * 0.84 * 0.44 −0.12
20 min
Viability (%) 0.93 * 0.95 * 0.66 0.22
Acrosome integrity (%) 0.96 * 0.95 * 0.65 0.23
Mitochondrial activity (%) 0.95 * 0.94 * 0.66 0.24
Motile sperm (%) 0.92 * 0.91 * 0.68 0.31
Progressive sperm (%) 0.94 * 0.93 * 0.68 0.22
30 min
Viability (%) 0.94 * 0.94 * 0.91 * 0.69
Acrosome integrity (%) 0.97 * 0.96 * 0.90 * 0.70
Mitochondrial activity (%) 0.97 * 0.95 * 0.89 * 0.69
Motile sperm (%) 0.96 * 0.93 * 0.88 * 0.70
Progressive sperm (%) 0.966 * 0.945 * 0.881 * 0.684
40 min
Viability (%) 1.00 * 1.00 * 0.89 * 0.81 *
Acrosome integrity (%) 1.00 * 1.00 * 0.89 * 0.80 *
Mitochondrial activity (%) 1.00 * 1.00 * 0.87 * 0.78 *
Motile sperm (%) 1.00 * 1.00 * 0.85 * 0.78 *
Progressive sperm (%) 1.00 * 1.00 * 0.86 * 0.77 *
50 min
Viability (%) 1.00 * 1.00 * 0.90 * 0.82 *
Acrosome integrity (%) 1.00 * 1.00 * 0.91 * 0.82 *
Mitochondrial activity (%) 1.00 * 1.00 * 0.88 * 0.79 *
Motile sperm (%) 1.00 * 1.00 * 0.87 * 0.77 *
Progressive sperm (%) 1.00 * 1.00 * 0.89 * 0.78 *
60 min
Viability (%) 1.00 * 1.00 * 0.91 * 0.83 *
Acrosome integrity (%) 1.00 * 1.00 * 0.92 * 0.83 *
Mitochondrial activity (%) 1.00 * 1.00 * 0.90 * 0.81 *
Motile sperm (%) 1.00 * 1.00 * 0.88 * 0.79 *
Progressive sperm (%) 1.00 * 1.00 * 0.91 * 0.80 *

WST-8 reduction rate = ΔOD at 450 nm. Data presented are the average values collected from 36 samples from 6 independent experiments, for each value, 6 measurements (n = 6) were performed. Asterisks and bold font (*) indicate that the positive correlation coefficient value differs significantly (p < 0.01) from zero.