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. 2020 Dec 4;9(12):1707. doi: 10.3390/plants9121707

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Relationships among the ∆Ct value, cDNA fragment size and seed aging time as revealed by qPCR analysis of canola seeds. (a) The relationship between the ∆Ct values of stored mRNAs of five canola genes and seed aging time. cDNAs of control and five acceleratedly aged seed samples for variable aging days were used. The ∆Ct values between the aged and control samples for Bn11_1490bp, Bn12_1500bp, Bn13_1486bp, Bn14_1500bp, and Bn17_1500bp (the fragment length analyzed is indicated by the subscript) were determined. (b) Relationship between the ∆Ct value and cDNA fragment size of canola gene Bn12. cDNAs of the control (100% germination) and 30-day AA seeds were used to obtain ∆Ct values of the fragments on gene B12; the fragments were Bn12_148bp, Bn12_487bp, Bn12_850bp, Bn12_1169bp, and Bn12_1500bp. (c) Correlation of the ∆Ct value with seed aging time analyzed using five cDNA fragments of Bn12. cDNAs of the control and five AA seed lots under variable aging days were used; the five fragments in Figure 4b were used. The linear regressions had at least a p < 0.05 in the F-test for each of the regressions.