Figure 5.

Estimated β values for one stored mRNA gene in Arabidopsis seeds aged at different temperatures and days. Seeds were treated at 22, 30, 33, 37, and 40 °C for 8 or 16 days, with seeds stored at 4 °C used as the unaged control. The β value was estimated as described in the Materials and Methods using the ∆Ct value for the 2000bp fragment of Arabidopsis gene At1g74310; this gene was previously assayed by Zhao et al. [45]. Each β value was obtained from three technical replicates.