Table 2.

Estimated β values for four wheat mRNA fragments of similar length in the AA seeds of different aging times.

Fragment	β Value (Break per Nucleotide per Day × 10−4)
	Day 6	Day 12	Day 18	Day 24	Average	Overall
W2950bp	3.30 ± 0.10	2.68 ± 0.04	2.62 ± 0.06	3.14 ± 0.18	2.94 ± 0.15	2.26 ± 0.51
W3950bp	1.86 ± 0.22	1.49 ± 0.11	1.71 ± 0.16	2.46 ± 0.36	1.88 ± 0.18
W10955bp	2.12 ± 0.15	1.94 ± 0.03	2.15 ± 0.02	2.42 ± 0.11	2.16 ± 0.09
W12950bp	1.80 ± 0.31	1.99 ± 0.25	2.41 ± 0.06	2.07 ± 0.02	2.07 ± 0.11
Average	2.27 ± 0.30	2.03 ± 0.21	2.22 ± 0.17	2.52 ± 0.19

Note: The ∆Ct values between the control and aged seeds, as shown in Figure 3a, were used to calculate the β values using Equation (4) in the Materials and Methods. The β value was estimated from each of three technical replicates and its standard error was shown. W2_950bp, for example, means that a 950 bp fragment of W2 mRNA (Table S2) was analyzed.