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. 2020 Dec 3;9(12):2595. doi: 10.3390/cells9122595

Figure 5.

Figure 5

FUS (129 W/cm2, 40 s) or FUS-Cav (1136 W/cm2, 40 s) demonstrated the additive effect to HT (45 °C, 30 min). (a) Clonogenic survival of FaDu, T98G, and PC-3 cells was evaluated after HT, FUS, and FUS-Cav treatment. (b) Relative cell metabolic activity of PC-3 cells measured with WST-1 assay 48 h post-treatment. (c) Semi-quantitative result of the Transwell® assay indicating cell invasive potential of PC-3 cells. (d) Representative microscopy images of Transwell® assay 48 h post-treatment. Scale bar = 100 µm. Data were normalized to the untreated control, which were set as 100% and relative values presented as mean ± SEM, n = 6, * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001.