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. 2020 Dec 4;11(12):1455. doi: 10.3390/genes11121455

Figure 2.

Figure 2

CLSM images of gametophyte-to-sporophyte transition and embryo development in Ceratopteris richardii. (A) Antheridia in a male gametophyte at 15 dps. (B,C) Archegonia in a hemaphrodite gametophyte at 15 dps. (D) Archegonial necks are reached by mobile sperms. (E–G) Early developmental stages of the embryo: two-celled embryo (E); epibasal division in an octant embryo (F); hypobasal division in an octant embryo (G). (H–O) The RAC is specified early in embryo development and a root primordium begins to form. Additionally, the first leaf grows until breaking the calyptra. (P–T; P′–S′). Other components are also developing: shoot apical meristem (P,Q); first root primordium with a root cap (P′,Q′). Sporophytes continue to develop and new organs emerge (R–T). Additionally, the first SBR begins to grow and the whole root body develops; all cell layers recognizable (R′,S′). Scale bars: 20 μm. White—Calcofluor White; Red—Propidium Iodide.