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. 2020 Dec 4;9(12):446. doi: 10.3390/biology9120446

Figure 3.

Figure 3

Expression level of analyzed proteins of adherens junctions (E-cadherin), desmosomes (desmoglein-2) and gap junctions (connexin-43) in SKOV-3 and SKOV-3.ip cells cultured in monolayer and 3D in vitro models. (A,C,E) The distributions of SKOV-3 cells (left plot) and SKOV-3.ip cells (right plot) according to fluorescence intensity detected after staining with E-cadherin-specific, desmoglein-2-specific and connexin-43-specific antibodies (red—monolayer culture, blue—spheroids, green—collagen hydrogel); (B,D,F) Levels of E-cadherin, desmoglein-2 and connexin-43 in monolayer and 3D models denoted as relative fluorescence values, calculated as a ratio of mean fluorescence intensity of cells stained with specific antibodies to mean fluorescence intensity of cells stained with antibodies of isotypic control. mnlr, monolayer; sph, spheroids; clgn, collagen hydrogel. “*” indicates significant difference in RF level from monolayer culture (ANOVA, Holm–Sidak’s multiple comparisons test, p < 0.05).