Figure 3.

Targeted irradiation with a collimated carbon-ions microbeam on blastula embryo of medaka. The center part of blastoderm (diameter: 500 μm) was irradiated with a collimated carbon-ions microbeam with various diameters (70, 120, and 180 μm) and doses (5, 10, 50 and 75 Gy) (A). Yolk was irradiated with 5 and 10 Gy microbeam with 180 μm diameter (B). The targeting irradiated areas are surrounded by red line in the pictures. The hatchability of blastula embryos irradiated with microbeam are summarized in (C).