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. 2020 Dec 5;9(12):2615. doi: 10.3390/cells9122615

Figure 2.

Figure 2

Representative T lymphocytes maturation gating strategy in coronavirus disease 19 (COVID-19) patients. (A) T lymphocytes gating strategy: FSC-A vs. FSC-H plot: Gating the cells that have an equal area and height, thus removing clumps (greater FSC-A relative to FSC-H) and debris (very low FSC), CD45 vs. SSC-A plot: Broad selection of lymphocytes based on their SSC/CD45 properties, CD3 vs. SSC-A plot: Broad selection of lymphocytes T based on their SSC/CD3 properties. CD4 vs. CD8 plot: Broad selection of lymphocytes T CD4+ (pink) and CD8+ (blue) based on their CD4/CD8 properties. (B) CD4+ T lymphocytes maturation gating strategy for each maturation subsets. CD62L vs. CD45RA plot and CD45RA vs. CD31: Broad selection of CD4+ T lymphocytes based on their CD62L/CD45RA and CD45RA vs. CD31 properties and allow select recent thymic emigrants T CD4+ cells RTE (plot 1: RTE CD4+ and plot 2: RTE CD4+), CD197 vs. CD45RO plot: Broad selection of CD4+ T lymphocytes based on their CD197/CD45RO properties: naïve CD4+ T cells, effector CD4+ T cells, central memory CD4+ T cells and effector memory CD4+ T cells. (C) CD8+ T lymphocytes maturation gating strategy for each maturation subsets. CD62L vs. CD45RA plot and CD45RA vs. CD31: Broad selection of CD8+ T lymphocytes based on their CD62L/CD45RA and CD45RA vs. CD31 properties and allow selected recent thymic emigrants T CD8+ cells RTE (plot 1: RTE CD8+ and plot 2: RTE CD8+), CD197 vs. CD45RO plot: Broad selection of CD8+ T lymphocytes based on their CD197/CD45RO properties: Naïve CD8+ T cells, effector CD8+ T cells, central memory CD8+ T cells and effector memory CD8+ T cells.