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Perfusion of the blood vessel in a brain slice with artificial cerebrospinal fluid (ACSF). Photomicrograph of a blood vessel in the living brain slice under normal conditions (A) and when pressure is applied inside the vessel (B). (C) Blood vessel with microcapillary inserted inside and the neuron and astrocyte with attached microelectrodes. A glass electrode was inserted inside the blood vessel in a 400 μm brain slice preparation, and 20–30 torr pressure (see text) was applied.
