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. 2020 Dec 7;12(12):3666. doi: 10.3390/cancers12123666

Figure 1.

Figure 1

(A) The expression of yes-associated protein (YAP) was regulated by never in mitosis gene A (NIMA)-related kinases (NEK) activity and its upstream kinase tousled-like kinase (TLK). Overexpression of wt-NEK1 resulted in elevated YAP expression and conversely in its degradation in LNCaP cells overexpressing the dominant negative mutant NEK1-T141A. Thioridazine (THD) led to degradation of YAP in parental LNCaP cells, even after treatment with bicalutamide (BIC), which led to overexpression of TLK1B. (B) YAP interacted with NEK1 and was enriched upon co-immunoprecipitation. TLK1 inhibition with 10 µM THD did not affect NEK1 interaction with YAP, and thus the state of NEK1 kinase activity did not affect YAP binding. (C) The expression of YAP was decreased in NT1 cells treated with two different inhibitors of TLK (THD and J54), with a corresponding increase in CL-YAP products. (D) Expression of several typical YAP target genes in LNCaP cells treated with THD.