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. 2020 Dec 6;9(12):1026. doi: 10.3390/pathogens9121026

Figure 1.

Figure 1

Phylogenetic trees based on the ORF (open reading frame) nucleotide sequence of the (a) VP7 and (b) VP4 genes of G1P[8] strains circulating in Mozambique and global strains obtained from GenBank. The trees were constructed based on the maximum likelihood method implemented in MEGA X [29], applying the best-fit nucleotide substitution model Tamura-3-parameter (T92+G+I) for VP7 and General Time Reversible (GTR-G) for VP4, determined by JModelTest [30]. Bootstrap values (1000 replicates) ≥70% are shown with DS-1 serving as an out-group (not shown in the final tree). Scale bar indicates genetic distance expressed as the number of nucleotide substitutions per site. Pre-vaccine Mozambican strains are indicated by blue squares, post-vaccine by red circles, the Rotarix® vaccine strain by a green triangle and Mozambican strains from previous studies [14,19] are indicated by black triangles. Lineages are defined from I-VIII for VP7 and I-IV for VP4 [21,22,23,25,26,27,28].