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. 2020 Dec 7;10(12):331. doi: 10.3390/life10120331

Figure 4.

Figure 4

Confirmation of DEPs related to PD pathologies. (a) The same amounts of total proteins prepared from WT, LK2GS#1- and LK2GS#2-pNSC were subjected to Western blot analysis with oxidative stress-related antibodies including PRDX2, PRDX5, PRDX6, HSPH1, HSPA4, and HSP27. GAPDH was employed as a loading control. (b) Quantification of protein expression levels after normalization using GAPDH. (c) Western blot confirmation of the cell adhesion, ECM related proteins, including TLN1/2, CFL1, FBLN1, ITGA3B1, ITGB1 and ARP2/3, and cytoskeleton molecules, including MYH11, SETP2, and ACTN-1. GAPDH was employed as a loading control. (d) Histograms showing the fold changes of their relative expression levels. Results are means ± S.E.M for three independent experiments. * p < 0.05, ** p < 0.01, compared with WT-pNSC.