Table 1.
Identification of differentially expressed protein in WT-pNSC and LK2GS-pNSC.
| Acession no. | Protein Name | MW (kDa) |
pI * Value | Coverage (%) † | Matched Peptides | Score | Fold Change (LK2GS/WT) | |
|---|---|---|---|---|---|---|---|---|
| WT | LK2GS | |||||||
| Oxidoreducates activity | ||||||||
| P32119 | Preoxiredoxin-2 (PRDX2) | 21.9 | 5.97 | 34.34 | 11 | 73.3 | 48.2 | 0.66 |
| P30044 | Peroxiredoxin-5 (PRDX5) | 22.1 | 8.7 | 25.7 | 3 | 8.38 | 3.92 | 0.47 |
| P30041 | Peroxiredoxin-6 (PRDX6) | 25 | 6.38 | 23.66 | 3 | 18.55 | 5.8 | 0.31 |
| Q92598 | Heat shock protein 105 (HSPH1) | 96.8 | 5.39 | 12.24 | 7 | 13.13 | 6.5 | 0.5 |
| P34932 | Heat shock 70 kDa protein 4 (HSPA4) | 94.3 | 5.19 | 12.98 | 7 | 24.6 | 14.56 | 0.6 |
| Q9BRX8 | Redox-regulatory protein FAM213A (F213A) | 25.7 | 8.84 | 6.55 | 1 | 4.65 | 0 | WT unique |
| Q9NRD8 | Dual oxidase 2 (DUOX2) | 175.3 | 7.85 | 1.03 | 1 | 0 | 3.4 | LK2GS unique |
| Q6DKJ4 | Nucleoredoxin (NXN) | 48.4 | 4.97 | 3.68 | 1 | 2.33 | 0 | WT unique |
| P21266 | Glutathione S-transferase Mu 3 (GSTM3) | 26.5 | 5.54 | 7.65 | 1 | 2.99 | 0 | WT unique |
| P10599 | Thioredoxin (TXN) | 11.7 | 4.9 | 8.57 | 1 | 1.71 | 0 | WT unique |
| Q96SL4 | Glutathione peroxidase 7 (GPX7) | 21 | 8.27 | 15.51 | 1 | 0 | 2.3 | LK2GS unique |
| Q8N543 | 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 1 (OGFD1) | 63.2 | 5.11 | 4.06 | 1 | 2.41 | 0 | WT unique |
| Q9BUT1 | 3-hydroxybutyrate dehydrogenase type 2 (BDH2) | 26.7 | 7.65 | 19.59 | 3 | 4.1 | 6.3 | 1.5 |
| Q9C0B1 | Alpha-ketoglutarate-dependent dioxygenase FTO (FTO) |
58.2 | 5.22 | 6.35 | 2 | 10.3 | 3.9 | 0.37 |
| P31150 | Rab GDP dissociation inhibitor alpha (GDIA) | 50.6 | 5.14 | 21.03 | 8 | 88.02 | 35.94 | 0.41 |
| P50395 | Rab GDP dissociation inhibitor beta (GDIB) | 50.6 | 6.47 | 39.1 | 15 | 130.3 | 61.6 | 0.47 |
| Q15738 | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL) | 41.9 | 8.06 | 6.43 | 1 | 2.6 | 0 | WT unique |
| Q8N183 | Mimitin, mitochondrial (MIMIT) |
19.8 | 8.98 | 3.55 | 1 | 1.93 | 0 | WT unique |
| P28331 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUS1) |
79.4 | 6.23 | 20.2 | 1 | 2.16 | 0 | WT unique |
| O60701 | UDP-glucose 6-dehydrogenas (UGDH) | 55 | 7.12 | 14.57 | 4 | 4.65 | 2.71 | 0.58 |
| P20674 | Cytochrome c oxidase subunit 5A, mitochondrial (COX5A) |
16.8 | 6.79 | 31.33 | 2 | 17.12 | 6.6 | 0.39 |
| Q9UDR5 | Alpha-aminoadipic semialdehyde synthase, mitochondrial (AASS) | 102.1 | 6.61 | 1.94 | 1 | 5.86 | 1.94 | 0.33 |
| P43304 | Glycerol-3-phosphate dehydrogenase, mitochondrial (GPDM) | 80.8 | 7.69 | 4.26 | 1 | 2.33 | 0 | WT unique |
| P40926 | Malate dehydrogenase, mitochondrial (MDHM) | 35.5 | 8.69 | 35.8 | 9 | 46.65 | 11.41 | 0.24 |
| Cell adhesion molecule binding | ||||||||
| Q9Y490 | Talin-1 (TLN1) | 269.6 | 6.07 | 5.51 | 5 | 26.58 | 14.37 | 0.54 |
| Q9Y4G6 | Talin-2 (TLN2) | 271.4 | 5.57 | 1.06 | 1 | 2.24 | 0 | WT unique |
| P23528 | Cofilin-1 (CFL1) | 18.5 | 8.09 | 44.58 | 6 | 95.82 | 46.26 | 0.48 |
| Q92616 | Translational activator GCN1 (GCN1L) | 292.6 | 7.46 | 2.28 | 3 | 10.01 | 3.84 | 0.38 |
| P04075 | Fructose-bisphosphate aldolase A (ALDOA) | 39.4 | 8.09 | 26.1 | 8 | 39.43 | 15.43 | 0.39 |
| P06733 | Alpha-enolase (ENOA) | 434 | 7.39 | 48.62 | 17 | 232.4 | 106.1 | 0.47 |
| P26038 | Moesin (MOES) | 67.8 | 6.4 | 13 | 8 | 45 | 22.64 | 0.5 |
| Q13813 | Spectrin alpha chain, non-erythrocytic 1 (SPTN1) | 284.4 | 5.35 | 1.94 | 3 | 1.53 | 0 | WT unique |
| Cytoskeleton molecules | ||||||||
| P35749 | Myosin-11 (MYH11) | 227.2 | 5.5 | 1.67 | 3 | 6.65 | 1.81 | 0.27 |
| Q15019 | Septin-2 (SEPT2) | 41.5 | 6.6 | 20.78 | 4 | 20.12 | 7.3 | 0.36 |
| P12814 | Alpha-actinin-1 (ACTN1) | 103 | 5.41 | 2.47 | 2 | 9.02 | 0 | WT unique |
| P62873 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (GBB1) | 37.4 | 6 | 6.18 | 2 | 5.76 | 3.73 | 0.65 |
| P62158 | Calmodulin (CALM) | 16.8 | 4.22 | 46.98 | 3 | 26.26 | 14.79 | 0.56 |
| P37231 | Peroxisome proliferator-activated receptor gamma (PPARG) |
57.6 | 5.94 | 3.37 | 1 | 1.88 | 0 | WT unique |
| DNA damage response | ||||||||
| P13010 | 86 kDa subunit of Ku antigen (XRCC5) | 82.7 | 5.81 | 19.67 | 10 | 69.55 | 31.34 | 0.45 |
* The isoelectric point of the intact protein. † The percentage of matched sequence in the total protein sequence (the percentage of the database protein sequence covered by matching peptides).