Figure 4.

Viability and integrity of PsA after ZnPor treatment. PsA grown overnight in MSG were diluted in fresh MSG to 0.15 OD. ZnPor was added at a concentration of 32 µg/mL; control was without ZnPor. Samples were measured at various time intervals (x-axis). PsA cells were washed to remove extracellular ZnPor. The same cell suspensions were sampled for all three measurements. (A) Total cell counts (cells/mL) were calculated (Petroff–Hausser cell-counting chamber). (B) Viable cell counts (CFU per mL) were determined by plating the same cells on LB agar medium. (C) Cell suspensions (from A,B) were measured (at 600 nm) to determine whether cells had lysed or remained intact. All data points represent mean ± SD of three independent experiments (n = 9).