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. 2020 Dec 7;18(12):624. doi: 10.3390/md18120624

Figure 1.

Figure 1

Amentadione (YP) reduces the levels of inflammatory markers in articular-derived cells stimulated with IL-1β (a,b). Primary chondrocytes and synoviocytes were pre-treated with 10 μM YP for 24 h, followed by stimulation with 10 ng/mL IL-1β during different time points. (a) Relative gene expression of the inflammatory marker COX-2 was determined by qPCR, at 3 h and 6h post IL-1β stimulation in chondrocytes and synoviocytes. (b) Levels of IL-6 in cell culture media 6 h post IL-1β stimulation, determined by ELISA. Cells treated with 2 μM dexamethasone (DXM) were used as a positive anti-inflammatory control. Data are presented as means of at least three independent experiments. All graphs show mean ± SD. One-way ANOVA and multiple comparisons were achieved with the Dunnett’s test. Statistical significance was defined as p ≤ 0.05 (*), p ≤ 0.005 (**) and p ≤ 0.0005 (***).