Figure 6.
Effects of oxidative stress on Δhex1 and its involvement in reactive oxygen species (ROS) metabolism. (A) Responses of the wild-type, the deletion (Δhex1), and the complemented (hex1-c) strains to H2O2, paraquat, iprodione, and N-acetyl cysteine with regard to germination (top; the number of inoculated conidia per spot are provided above the images; growth for 3 days) and radial growth (bottom; growth for 18 days). CM: CzD-CM. (B) Relative growth inhibition by the same substances (each condition was tested in triplicate; bars = SD; statistical testing by one-way ANOVA followed by Tukey’s post hoc test (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001). All concentrations are given in mM, except for iprodione (μg/mL). (C) Detection of superoxide anion radicals (O2−) using nitro blue tetrazolium chloride (NBT) staining in colonies of the two strains upon treatment with various sources of stress (NaCl, sorbitol, paraquat, and farnesol). Both top and bottom views of plates are shown for every treatment.