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. 2020 Dec 7;6(4):344. doi: 10.3390/jof6040344

Figure 7.

Figure 7

Vdhex1 is not involved in heterokaryon incompatibility. (A) Time-lapse microscopic examination of a fusion event between the incompatible strains BB Δhex1 H1-sGFP and Ls.17 sGFP. Typical characteristics of incompatibility-induced cell death, namely, nuclear degradation (arrowheads) and cell shrinkage (asterisks), are observed. Septae that surround the fused cells are indicated by numbers 1–4, and two control septae in neighboring hyphal compartments are indicated by C1–C2 (see also Figure S5, Supplementary Materials). Cell wall staining was performed using calcofluor white M2R. Bar = 10 μm. (B) Quantification of blue fluorescence intensity (calcofluor white M2R staining of cell wall chitin) in the region of interest shown in panel A, for two post-fusion time points, before and after the visible effects of the incompatibility reaction.