Table 3.
Days of Ripening | 0 | 2 | 5 | 9 | 14 | 21 | 30 | SEM | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CNT | EQU | SAP | CNT | EQU | SAP | CNT | EQU | SAP | CNT | EQU | SAP | CNT | EQU | SAP | CNT | EQU | SAP | CNT | EQU | SAP | ||
Total aerobic mesophilic bacteria | 5.48 a,1 | 6.54 a,2 | 6.57 a,2 | 5.93 b,1 | 6.75 b,2 | 7.24 b,3 | 6.89 c,1 | 7.93 c,2 | 7.88 c,2 | 8.37 d,1 | 8.78 d,2 | 8.99 d,3 | 8.62 e,1 | 8.97 e,2 | 9.08 e,2 | 8.78 f,1 | 9.06 f,2 | 9.19 f,2 | 9.00 g,1 | 9.12 g,2 | 9.19 f,2 | 0.16 |
Staphylococci | 4.82 a,1 | 6.26 a,2 | 6.42 a,2 | 4.71 b,1 | 6.31 a,2 | 6.75 b,3 | 5.86 c,1 | 6.90 b,2 | 6.59 a,b,3 | 6.40 d,1 | 7.63 c,2 | 7.53 c,2 | 6.33 e,1 | 8.05 d,2 | 7.81 d,3 | 6.24 f,1 | 8.13 d,2 | 7.95 d,2 | 6.29 g,1 | 8.08 d,2 | 7.89 d,2 | 0.15 |
Lactic acid bacteria | 4.12 a,1 | 5.47 a,2 | 5.30 a,3 | 4.96 b,1 | 6.15 b,2 | 5.80 b,3 | 5.42 c,1 | 6.36 c,2 | 7.12 c,3 | 7.35 d,1 | 7.68 d,2 | 7.86 d,3 | 8.40 e,1 | 8.98 e,2 | 9.00 e,2 | 8.37 f,1 | 9.01 f,2 | 9.15 f,3 | 8.60 g,1 | 9.14 g,2 | 9.23 f,2 | 0.14 |
Enterobacteriaceae | 2.98 a,1 | 2.97 a,1 | 3.09 a,2 | 3.56 b,1 | 3.02 a,2 | 3.28 b,3 | 4.77 c,1 | 4.91 b,2 | 4.84 c,1,2 | 5.42 d,1 | 5.69 c,2 | 5.36 d,1 | 5.78 e,1 | 5.47 d,2 | 5.52 e,2 | 5.00 f,1 | 4.47 e,2 | 4.62 f,3 | 3.93 g,1 | 3.59 f,2 | 3.37 b,3 | 0.15 |
CNT: Non-inoculated control batches; EQU: Batches inoculated with L. sakei + S. equorum; SAP: Batches inoculated with L. sakei + S. saprophyticus. a–g Means in the same row and sausage group (CNT, EQU or SAP) not followed by a common letter differ significantly (p < 0.05) (differences associated to the ripening time). 1–3 Means in the same row and ripening time not followed by a common number differ significantly (p < 0.05) (differences associated to the use of starter cultures). SEM: standard error of the mean.