Table 1.
Advantages and disadvantages of drug quantification techniques. UV-Vis: Ultraviolet-Visible; ELISA: Enzyme-linked immunosorbent assay; HPLC: High-performance liquid chromatography; MS: Mass spectrometry; RT-qPCR: Real-time quantitative polymerase chain reaction. +: Low, ++: Medium, +++: High.
| Criteria | UV-Vis Absorbance | Fluorescence | ELISA | HPLC-UV | HPLC MS | (RT)-qPCR |
|---|---|---|---|---|---|---|
| Convenience | +++ | +++ | +++ | ++ | + | + |
| Low cost | +++ | +++ | ++ | ++ | + | + |
| Complexity | + | + | + | ++ | +++ | +++ |
| Sensitivity | + | ++ | ++ | ++ | +++ | +++ |
| Selectivity | + | ++ | +++ | ++ | +++ | +++ |