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. 2020 Dec 8;8(12):582. doi: 10.3390/biomedicines8120582

Figure 3.

Figure 3

Verbascoside reduces ER stress. (A) Western blot analysis of ER stress markers in cells treated with 16 μM verbascoside (VB) for 5 days (30 μg protein/sample). On the right, the molecular-weight size markers in kDa are reported. (B) The quantitative analysis shows that verbascoside treatment reduces the expression of HSP70, BIP and PERK proteins. Data (mean values ± SD) are expressed as fold change over control (dashed line). (n = 3–5 independent experiments performed in triplicate) (Student’s t-test * p < 0.05 vs. Ctr). (C) Mouse βtc3 cells were incubated with 16 μM verbascoside (VB) for 5 days and ER stress was induced by 2 μg/mL tunicamycin treatment for 7 h. MTT test reveals a protective role of verbascoside against the tunicamycin-induced ER stress. Data are expressed as mean ± SD of three independent experiments (two-way ANOVA, post-hoc Tukey’s test. °°° p < 0.0001 tunicamycin vs. Basal; * p = 0.024 VB vs. Ctr; *** p = 0.0001).