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. 2020 Nov 16;12(23):23960–23973. doi: 10.18632/aging.104073

Table 1. Primers used in polymerase chain reaction (PCR) assay.

Gene Primer sequence (5’→3’)
lncHCG18 full length on Plasmid Forward GAATTCTTAGAGGATCCTGTTT
Reverse TCTAGATTTTTAGAAAGATGAT
SOX7 CDS full length on Plasmid Forward CTGCTGGGAGCCTACCCTTGG
Reverse CAGCCAGGACGGAGATGAG
lncHCG18-244 Forward GAATTCTTAGAGGATCCTGTTT
Reverse TCTAGATTTTTAGAAAGATGAT
SOX7 3’UTR Forward GTTGATGATATGATTGACTGATGC
Reverse CTGTTCCAAAGTATGAGTTGTTCT
SOX7 3’UTR mut Forward CATAAAACAGTATCACAGACATGCCGTGGAACTC
Reverse TTCAAAAAAACGAAACAGTTCCACGGCATG
VE-Cadherin promoter Forward AGGAGGGTTAAATGTGATGCTG
Reverse GGGATGTTTCTGTTCCGTTG
VE-Cadherin promoter mut Forward CAAAGGCGACCCAACAGGAA
Reverse CCTGTTGGGTCGCCTTTGT