Table 1. Primers used in polymerase chain reaction (PCR) assay.
Gene | Primer sequence (5’→3’) | |
lncHCG18 full length on Plasmid | Forward | GAATTCTTAGAGGATCCTGTTT |
Reverse | TCTAGATTTTTAGAAAGATGAT | |
SOX7 CDS full length on Plasmid | Forward | CTGCTGGGAGCCTACCCTTGG |
Reverse | CAGCCAGGACGGAGATGAG | |
lncHCG18-244 | Forward | GAATTCTTAGAGGATCCTGTTT |
Reverse | TCTAGATTTTTAGAAAGATGAT | |
SOX7 3’UTR | Forward | GTTGATGATATGATTGACTGATGC |
Reverse | CTGTTCCAAAGTATGAGTTGTTCT | |
SOX7 3’UTR mut | Forward | CATAAAACAGTATCACAGACATGCCGTGGAACTC |
Reverse | TTCAAAAAAACGAAACAGTTCCACGGCATG | |
VE-Cadherin promoter | Forward | AGGAGGGTTAAATGTGATGCTG |
Reverse | GGGATGTTTCTGTTCCGTTG | |
VE-Cadherin promoter mut | Forward | CAAAGGCGACCCAACAGGAA |
Reverse | CCTGTTGGGTCGCCTTTGT |