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Percentage of HKa* binding to tropomyosin at different pH. HKa* (80 nM) in MOPS pH 7.5 (a) or pH 8.0 (b), in presence or not of 25 uM of TetraHPRG and in presence or not of CuSO4 or ZnCl2 (10 µM). Data are the mean ± SEM of three different experiments performed in triplicate. Statistically significant differences are indicated with *** = p < 0.001 and **** = p < 0.0001 vs. HKa 80 nM (One-Way ANOVA + Dunnett’s test).
