Figure 8.

% Binding of HKa* to plate previously coated with tropomyosin (left group) or not (right). After tropomyosin the plate has been blocked with milk 5% and the pretreated with TetraHPRG at 25 µM before to the addition of HKa* (80 nM) in MOPS pH 7.5 in presence or not of CuSO₄ or ZnCl₂ (10 µM). Data are the mean ± SEM of three different experiments performed in triplicate. Statistically significant differences are indicated with **** = p <0.0001 vs. HKa 80 nM (One-Way ANOVA + Dunnett’s test).