Figure 2.

Expression of the glucose transporters GLUT1, GLUT2, and GLUT3 in the non-irradiated uveal melanoma (UM) samples. (A) Representative images from the immunostainings for GLUT1-3. The disomy-3 tumor was obtained from a male patient (69 years), who did not develop metastases during the follow-up, whereas the monosomy-3 tumor was removed from a female patient (39 years), who presented with bone metastases that have subsequently spread to the brain. Images were acquired at an original magnification of 200×. The black arrows indicate the pigmentation whereas the white arrows with a dark contour point out the staining of the plasma membrane. Scale bar = 25 μm. (B) Immuno-Fluorescent in situ hybridization (Immuno-FISH) was performed to detect the copy number of chromosome-3 (red) in the UM cells that were positive for the melanoma marker Melan-A (green). The nuclei were counterstained in blue with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI). The chromosome 3 status of the tumors was evaluated by determining the percentage (%) of cells with monosomy-3 and the chromosome 3-index as described in the Methods section. The values obtained for these parameters in the previously mentioned tumors are stated underneath the respective image panels (n: number). Arrows indicate several examples of cells with monosomy-3. Scale bar = 10 μm. Quantification of the intensity and ratio of (C) GLUT1, (D) GLUT2, and (E) GLUT3 in the primary UMs with regard to the monosomy-3 status. (F) The sum of the GLUT1-3 intensities was also presented as the “total.” p-values were determined by the unpaired, two-sided t-test. The significant p-values (<0.05) were indicated in red. a.u.: Arbitrary units.